adamianis
qorionuli gonadotropinis (aqg) winaaRmdeg mimarTuli autoantisxeulebi reproduqciuli sistemis avTvisebiani da keTilTvisebiani simsivniT daavadebulebSi.
l.axvlediani, n.foraqiSvili, o.Caxoiani, j.qiqava.
baTumis
saxelmwifo universiteti, Tbilisis saxelmwifo universiteti, baTumis mezRvaurTa samedicino centri.
reziume
Cvens mier miRebuli monacemebiT
keTilTvisebiani simsivniT daavadebulebSi (sakvercxis histoma, endometriumis polipi,saSvilosnos fsevdomucinozuri
kista) anti-hCG
antisxeulebis Semcveloba sisxlis SratSi ufro maRal maCvenebels
iZleva avTvisebiani simsivnis mqone gamokvleul pirebTan SedarebiT (saSvilosnos tanis kibo, saSvilosnos
yelis kibo,sakvercxis kibo) es SesaZlebelia
miuTiTebs imaze, rom specifiuri antisxeulebis produqcia moqmedebs hCG-is neitralizaciaze da amitomac aferxebs simsivneTa zrdis efeqts. Cveni mosazrebiT anti-hCG
antisxeulebis aRmoCena SesaZlebelia gamodges araavTvisebiani simsivneebis diferencialuri diagnozisaTvis.
Sesavali
adamianis
qorionuli gonadotropini (aqg) glikoproteinul hormonTa ojaxis wevria. igi
Sedgeba arakovalenturad dakavSirebuli suberTeulebisagan.Tavisufali
hCGα da hCG β cilebi kodirdeba ramdenime damoukidebeli geniT [1,4,9,10].
glikoproteinul hormonTa
ojaxSi Sedis aseve maluTeinizirebeli Hhormoni(LH), folikulmastimulirebeli hormonida
Tiroid-mastimulirebeli hormoni.
yoveli maTgani
warmodgenilia
αβ heterodimeris saxiT[2]. qg
danarCeni hormonebisagan imiTgansxvavdeba, rom igi sinTezirdeba placentas trofoblasturi ujredebis mier ganayofierebis Semdeg da misi
aRmoCena SratSi SesaZlebelia orsulobis dros. qg-is zemoqmedebiT yviTeli sxeuli asinTezirebs iseTi raodenobiT progesterons, rom ar xdeba endometriumis
ukugdeba da narCundeba orsuloba. amis garda
nativuri glikoproteini da suberTeulebi aseve sinTezirdeba trofoblasturi da aratrofoblasturi warmoSobis malignizirebadi ujredebis mier [8]. gamokvlevebma aCvena,
rom aratrofoblasturi simsivnis mqone pirebSi xSirad Tavisufali hCG β-is done SratSi
aRwevs 100pg/ml-s[6,9]. Tavisufali hCG β-s deteqcia SratSi ufro meti sixSiriT
(30%-40%) gvxvdeba saSarde buStis, pakreasis, saSvilosnos yelis simsivneebis dros [6]. amitom Tavisufali hCG β-s deteqcia SratSi,
gansakuTrebiT mamakacebSi, SeiZleba CaiTvalos simsivneasocirebul markerad da misi
aRmoCena miuTiTebs daavadebis cud prognozze.
simsivneebis dros
SratSi hCG-is eqspresia SesaZlebelia ZiriTad rols TamaSobdes
specifiuri autoantisxeulebis gamomuSavebaSi,
rac saukeTeso serologiuri markeria.
masala da
meTodi
Cven gamovikvlieT 10 saSvilosnos tanis kiboTi daavadebulis, 10 sakvercxis kiboT daavadebulis, 10 endometriumis polipis mqone, 6 saSvilisnos yelis kiboTi daavadebulis, da 5 sakvercxis histomis mqone pirTa periferiuli sisxlis Srati. gamokvleul
pirTa asaki iyo 35-68 wlamde.
kvlevis mizans
warmoadgenda adamianis qorionuli gonadotropinis mimarT autoantisxeulebis titris dadgena arapirdapiri imunofermentuli analiziT.
96 fosoian brtyelZirian planSetebze vaxdendiT 50 mkl hCG (SIGMA) datanebas 1mg/ml koncentraciiT karbonat-bikarbonatul
buferSi da vainkubirebdiT 24sT. 4 gradusze. planSets
vrecxavdiT fosfaturi buferiT, romelic Seicavda 0,05% Tvin-20-s (SIGMA).
Semdgom fosoebSi vasxamdiT
sakvlev Srats, ganzavebuls tvinisa da 2% xaris Sratis
albuminis Semcveli fosfaturi buferiT (SIGMA). sakvlev
Srats vanzavebdiT 1:25,
1:50, 1:100, 1:200, 1:400, 1:800, 1:1600, 1:3200, 1:6400, 1:12800–jer.
koniugatis saxiT
viyenebdiT Tagvis anti-adamianis IgG-s,romelsac vamatebdiT
yvela fosoSi optimaluri titriT1:100.
substrates saxiT viyenebdiT 10 mlg p-nitrofenil
fosfatids, romelsac vxsnidiT 100mkl MgCI2-is Semcvelkarbonat-bikarbonatul
buferSi. reaqcias
vaCerebdiT 12% -iani gogirdmJavaTi.
optikur simkvrives
vsazRvravdiT multiskan MCC-ze,
talRis sigrZe-405 nm.
Sedegebi
anti-hCG antisxeulebis titri gamokvleul pacientebSi naCvenebia #1,2,3 suraTebze. yvelaze maRali
titri anti-hCG antisxeulebisa aRmoaCndaT sakvercxis histomiT daavadebul da saSvilosnos fsevdomucinozuri kistis mqone pirebSi.
yvelaze
dabali maCveneneblebiT xasiaTdeboda saSvilosnos
rasakvirvelia
hCG ZiriTadad sinTezirdeba trofoblasturi ujredebis mier orsulobis SesanarCuneblad. es
glikoproteini aseve sinTezirdeba malignizirebuli ujredebis mier rogorc trofoblasturi aseve ara trofoblasturi
simsivneebis dros.
hCG-is
Tavisufali β
suberTeuli farTod
gamoiyeneba, rogorc daunis sindromis markeri [11,3] ,aseve warmoadgens zogi epiTeluri simsivnis prognozirebad markers [5].
ufro metic hCG-is SratSi
aRmoCena SesaZlebelia reproduqciuli sistemis simsivneebis dros. am SemTxvevaSi, orsulobisagan gansxvavebiT hCG
sinTezirdeba simsivnuri ujredebis mier ufro meti vidre
Tavisufali hCGβ.
aris imis mtkicebuleba, rom hCGβ
TamaSobs simsivnis
zrdis mastimulirebeli hormonis rols. Tavisufali hCGβ-s eqspresia
solidur simsivneebTan miuTiTebs mis agresiulobaze,maRal metastazirebad potencialsa da swraf zrdaze[7]. sisxlis cirkulaciaSi yofnisas hCGβ molekulebis umetesoba simsivnis zrdis procesSi iwvevs organizmis imunizacias da anti-hCG antisxeulebis gamomuSavebas, rac warmoadgens Cveni kvlevis mizans.
References
1. Boorstein W.R., Vamvakopoulos
NK.,and Fiddes
JC. (1990). Human chorionic gonadotrophin
beta-subunit is encoded by at least eight genes arranjed
in tandem and inverted pains. Nature.300;419-422.
2. Butler S.A. Laider
P.,Porter J. R.,KicmanA.T>,Chard
T.,Covan D.A. and Iles
R.K.,(1999) β-subunit of human chorionic gonadotrophin exists as e homodimer.Journal
of Molecular Endokrinology,22:185-192.
3. Cucle H.S.Iles RK
and chard T.(1994). Urinari βcore hCG a new approach to Dovn’s syndrome screening.Prenatal
diagnosis 14;953-958.
4. FiddesJC and Goodman HM,(1980).
The cDNA for β-subunit of human chorionic gonadotrophin suggests
evolution of a gene by readthrough into 3’- untranslated region. Nature, 286;684-687.
5. Iles RK.,Persad
R.,Trivedi M.,Sharma KB.,Dickenson A.,Smith P. and
Chard T. (1996) Urinari concentration of hCG and its
fragments as a prognostic marker in bladder cancer. British Journal of Urology,
77;61-69.9
6.
7. Moutzoauris G, Yannopoulos
D, Barbatis C, 91993). Is beta human chorionic gonadotrophin
production by transitional cell carcinoma
of the bladder a marker for aggressive desease
and resistanse to chemotherapy? Br.J.Urol.72;807-809.
8. Pierse JG and Parsons TF(1981), Glikoprotein hormones;Strukture
and function. Annu.Rev Biochem.50;465-495.
9. Policastro P. Ovitt CE., Hoshina M, Fukuoka H. Boothby MR. and
10. Porakishvili N. Jakson AM.,
DE Souza JB, Dalla chiesa
M, Roitt IM,
Delves PJ, Lund T. Epitopes of human chorionic gonadotrophin and thear relationship to immunogenisity
and cross-reaktivity of beta chain mutants. Am. J. Repro.Immunol.
11998,40;210-214.
11. Spenser K. Coombs EJ. MALLARD as AND Milfordvard A, (1992). Free beta human chorionic
gonadotrophin in dovn’s
syndrome screening;a multicancer study of its role compared
with other biological markers , Annals of clinical biochemistry 29;
506-518.
Abstract
The patient with ovarian histoma and uterus cista
showed the highest titre
of anty-hCG autoantibodies
out of the patients studied.The patient with malignant tumours hed non-appreciable levels of anti-hCG
antibodies.
However a correct measurement of the
serum hCG β is
technically complex which limits its application for tumour
screening. the serum expression of hCG
in malignancies may lead to the
induction of specific autoantipodies,which can
represent a beeter serum marker.In
addition it will demonstrate a
possibility of a recognition of the tumor- associated antigens.
sakvercxis histoma
sakvercxis kibo
saSvilosnos
